Ultrastructure of Paneth cells in the intestine of various mammals

Paneth cells in the following species were observed under an electron microscope: human, rhesus monkey, hare, guinea pig, rat, nude rat, mouse, golden hamster, and insect feeder bat. Secretory granules containing homogeneous electron-dense materials were observed in the Paneth cells of humans, monkeys, hares, guinea pigs, and bats; mouse Paneth-cell granules were bipartite (central core and peripheral halo), and the Paneth cells in rats and golden hamsters had secretory granules showing various electron densities. In humans, monkeys, and bats, immature granules near the Golgi apparatus sometimes showed bipartite substructure. The number and size of secretory granules were also diverse among various animal species. Some lysosome-like bodies were commonly observed in peri- or supranuclear regions, though the size and shape of the bodies differed from cell to cell. In apical cytoplasm, small clear vesicles (100–200 nm diameter) were more-or-less observed in all species examined, and it was especially note that rat Paneth cells contained many clear vesicles. Small dense-cored vesicles (150–200 nm diameter) were rare. It is unlikely that the various ultrastructural features of Paneth cells correlate with the phylogenetical classification.     

Behaviour of soft granules under compression: Effect of reactive and non-reactive nature of the binder on granule properties

Granulation is a process where primary powder particles are made to adhere to form multi-particle entities called granules and this is achieved by using a binder. The binders can be broadly classified into two categories viz. reactive (reacts with base powder) and non-reactive (does not react with the base powder). The effect of various parameters related to binder liquid (binder viscosity, addition rate, distribution over the bed etc.) on the mechanism of granulation and physical/mechanical properties of granules is well studied. However, comparison of physical and mechanical properties of granules made via reactive and non-reactive binder using the same base primary particles has not been reported. In this paper, granulation of sodium carbonate primary particles under reactive and non reactive conditions was studied. The mechanical properties of sodium carbonate granules were characterized using single granule compression measurements. The average single granule apparent strength of reactive granules was higher compared to non-reactive granules. It was observed that granules formed using non reactive binder were brittle and showed multiple breakages. However granules made using reactive binder showed single breakage followed by significant plastic flow. In addition, bulk granule compression measurements were also carried out. Known models of Heckel, Kawakita and Ludde, and Adams et al. (developed mainly for pharmaceutical and metal powders) were used to predict mechanical properties of soft detergent granules. The bulk granule compression measurements also showed that reactive granules have higher strength compared to non-reactive granules. However, the absolute values of granule strength obtained from the empirical models were lower than the granule strength obtained from single granule compression measurements.     

The effect of increasing application rate of granular calcium ammonium nitrate on net nitrification in a laboratory study of grassland soils

Soil incubation studies were undertaken in controlled environment cabinets at 15°C to investigate the effect of increasing application rates of calcium ammonium nitrate (CAN) on net nitrification in two grassland soils. Granular CAN was applied to the surface of freshly collected, moist soil, at a rate equivalent to 0, 100, 200, 400, 800 and 1600µg NH ₄ ⁺ -N and NO ₃ ^- -N per gram of oven dry soil. In half the treatments finely ground CaCO₃ was incorporated into the moist soil to raise the starting pH. Changes in soil mineral N and pH were measured at weekly intervals up to six-weeks. The most probable number (MPN) technique was used to enumerate the NH ₄ ⁺ -N and NO ₂ ^- -N oxidizers at the beginning and end of the incubation.At low rates of CAN application there was considerable NH ₄ ⁺ -N oxidation to NO ₃ ^- -N during the incubation of both soils. Lime stimulated this N transformation. At high application rates (i.e. 800 and 1600 ppm) there was little change in NH ₄ ⁺ -N or NO ₃ ^- -N on either soil during the 6 week incubation, in the presence or absence of lime. The rate of NO ₃ ^- -N produced peaked at 5.6 and 3.8 mg NO ₃ ^- -N kg^–1 d^–1 on soil 1 and 2 respectively, in the presence of lime. Above a level of 400 ppm CAN (equivalent to 38 kg N ha^–1) the rate of NO ₃ ^- -N produced decreased. The higher rate of net nitrification in soil 1 compared with soil 2 was probably due to a higher number of nitrifying bacteria. Although high rates of CAN decreased the nitrifying activity of both soils there was little difference between treatments in the actual numbers of NH ₄ ⁺ -N and NO ₂ ^- -N oxidizers determined by the MPN technique.The results showed that the rate of granular CAN applied to the soil surface can influence the local activity of nitrifying bacteria and subsequent N transformations. At application rates of CAN generally used agriculturally for grass production, it is likely that net nitrification of the NH ₄ ⁺ -N in the fertilizer granule will be inhibited.     

A novel nucleation apparatus for regime separated granulation

A novel nucleation apparatus is presented for the production of narrow sized nuclei from various powder and binder liquid combinations. Mono-sized binder liquid droplets are produced by a specially designed mono-disperse droplet generator. The droplet generator is positioned above a conveyor belt, transporting a powder bed through the spray zone of the droplet generator. By nucleating powder on a conveyer belt, the nucleation mechanism is completely separated from all other granulation mechanisms due to the lack of relative motion between primary particles and/or formed nuclei. Nucleation tests were performed using chalcopyrite and limestone powders with water as the binder liquid. At all operating conditions, the formed nuclei were found to originate from multiplicities of drops that merged on the powder bed surface. Investigation of the dynamics of nuclei formation showed that powder-binder liquid combinations with fast penetration dynamics result in less variation in the number of droplets from which nuclei originate. Smaller and more narrowly distributed nuclei were also achieved by increasing powder speed through the spray zone.     

高温大颗粒气固流化床流化行为数值模拟

通过数值模拟,对内含直径为3 mm流化颗粒的二维高温流化床开展了研究。其中欧拉途径被用于描述气相运动,而对颗粒相描述则采用拉格朗日途径。采用离散单元法跟踪颗粒在不同时刻位置和速度。结果表明:与300K时相比,1 000 K高温床的临界流化风速有所提高,压降脉动及床层最大膨胀比明显降低。1 000 K时,流化数提高至1.67,高温床内可见到大尺寸的气泡。在气泡之外上、下2个区域中处于气泡对称轴上的颗粒,水平速度具有相反的方向;而y方向上,这二部分颗粒群的速度均向上并大致在10-1m/s范围之内。     

中间苍白杆菌对含蜡原油除蜡降粘分析

蜡质不仅使含蜡原油黏度升高，而且会析出积聚在管壁上降低管输效率。从原油污染淤泥中分离出一株产表面活性剂的烃降解菌F-1，经16S rDNA鉴定为中间苍白杆菌。与原油作用7 d 后，能将蜡含蜡量为15.2%的高含蜡原油处理为蜡含蜡量为9.1%的含蜡原油，原油蜡质量分数降低40.1%，原油黏度降低21%以上，细胞疏水性达28.1%。通过傅里叶红外光谱鉴定该菌产生的生物表面活性剂为脂肽类。添加该菌上清液到液体石蜡 可形成91.49 mm的排油圈，能够显著降低培养基表面张力，对液体石蜡乳化系数达到65%。     

固体颗粒介质成形新工艺及变形研究

固体颗粒介质成形新工艺,是采用固体颗粒代替刚性凸模(或弹性体、液体)的作用,对金属板料成形的工艺。固体颗粒介质成形新工艺,即可以解决流体介质、粘性介质的密封难题,又具有内压非均匀分布、便于控制成形、提高材料成形极限、降低投资成本、所得零件表面质量高、成品率高的优点,且固体颗粒无工业污染,可重复使用。该工艺为材料的加工制备提供了新的方法和手段。利用塑性增量理论,对自由变形区任意一点的应变进行了分析,得到了自由变形区任一点的应变及厚度计算公式。采用固体颗粒介质成形工艺,进行板料成形试验,成功试制出多种典型工件;对试验件壁厚分布的计算值和实测值进行了比较,证明理论正确。     

板材固体颗粒介质成形新工艺

提出了板材固体颗粒介质成形新工艺,即采用固体颗粒介质代替刚性凸模(或弹性体、液体)的作用对板料进行软模成形的工艺。通过成形试验,对板材固体颗粒介质成形新工艺的工艺过程、成形特点进行了论述,并进行了数值模拟。试验和模拟结果表明,应用固体颗粒介质成形工艺可以极大限度地发挥板料的成形性能,不需要任何辅助工序,可以一次成形深拉深件,且表面质量好,成品率高。     

颗粒介质传压性能的数值模拟和试验研究

文章作者自行研制了测试颗粒介质传压性能测试装置,通过试验,测得了多种颗粒介质在各种试验参数下的压力分布规律和应力应变关系。通过将颗粒介质作为连续体介质来处理,建立了研究颗粒介质传压性能的有限元模型,对不同试验参数下的颗粒介质,在承压筒底面上垂直压力分布进行了模拟计算。模拟和实测结果对比表明,应用连续体材料模型的模拟结果和实测结果较为接近。     

Modélisation de la cinétique de biodégradation de phénol par granules aérobies

This work describes a model of the biodegradation of phenol carried out by aerobic granules. These granules were obtained by culturing an activated sludge supernatant in a sequencing batch reactor fed with a synthetic waste water and subsequently, by acclimation to phenol (100 mg/L). The kinetics of phenol biodegradation by the aerobic granules was investigated over a wide range of initial phenol concentrations (40–1112 mg/L) in shake‐flask cultures. A Haldane‐type model was adjusted to the experimental results, which depicts successfully the phenol biodegradation profiles in the entire range of initial concentrations studied by using only one set of parameters. It is our view that the proposed model could contribute to the knowledge about the ability of aerobic granular systems to biodegrade toxic, inhibitory compounds such as phenol.